Primary objective: Does a 30-minute episode of helium breathing in humans decrease the amount of pro-inflammatory cytokines and chemokines produced in whole blood after immunostimulation ex vivo?
ID
Bron
Verkorte titel
Aandoening
immune response, helium
Ondersteuning
Onderzoeksproduct en/of interventie
Uitkomstmaten
Primaire uitkomstmaten
1. Proinflammatory cytokines in whole blood: Il-1beta, Il-6, TNF-alpha;<br>
2. Chemokine: Il-8, T-cell products Il-2, INF-gamma;<br>
3. Neutrophil degranulation product: elastase.
Achtergrond van het onderzoek
Various studies have shown protective effects of helium inhalation on ischemia/reperfusion damage in mycoardial and cerebral tissue. Based on these findings we want to investigate whether an extended episode of helium inhalation has any effect on the immune response. This is an explorative study with a cross-over design, using the principle of balanced assignment in a group of 12 healthy, male volunteers. The main endpoints are proinflammatory cytokines, chemokines, T-cell products and the neutrophil product elastase.
Doel van het onderzoek
Primary objective: Does a 30-minute episode of helium breathing in humans decrease the amount of pro-inflammatory cytokines and chemokines produced in whole blood after immunostimulation ex vivo?
Onderzoeksopzet
T0 = 15 mins before start of helium or room air breathing;
T1 = After 25 mins of helium/room air breathing;
T2 = 60 mins after helium/room air breathing;
T3 = Two hours after helium/room air breathing;
T4 = Six hours after helium/room air breathing;
T5 = 24 hours after helium/room air breathing.
Onderzoeksproduct en/of interventie
1. Helium or normal air breathing through a mask for 30 minutes;
2. Blood sampling through seperate puncturing sites.
Publiek
University of Amsterdam, Meibergdreef 9
Postbus 22660 H1Z-120
B. Preckel
Amsterdam 1100 DD
The Netherlands
+31 20 5662162,
b.preckel@amc.uva.nl
Wetenschappelijk
University of Amsterdam, Meibergdreef 9
Postbus 22660 H1Z-120
B. Preckel
Amsterdam 1100 DD
The Netherlands
+31 20 5662162,
b.preckel@amc.uva.nl
Belangrijkste voorwaarden om deel te mogen nemen (Inclusiecriteria)
Non-smoking, healthy, male volunteers, aged 18-45 years old.
Belangrijkste redenen om niet deel te kunnen nemen (Exclusiecriteria)
1. Smoking; current or in past six months;
2. Alcohol or drug abuse;
3. Allergic reaction to medication in past medical history;
4. Chronic medication or presence of any condition that could influence the immune system.
Opzet
Deelname
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