With a recently developed Ara h6 ELISA we would like to show the effect of exercise on circulating Ara h6 after peanut consumption
ID
Bron
Verkorte titel
Aandoening
Healthy volunteers, Food allergy, exercise, intestinal permeability, gezonde vrijwilligers, voedselallergie, inspanning, darmdoorlaatbaarheid
Ondersteuning
Onderzoeksproduct en/of interventie
Uitkomstmaten
Primaire uitkomstmaten
The main study parameter is the difference in serum Ara h6 levels over time between a resting and an exercise condition
Achtergrond van het onderzoek
In this intervention study 10 healthy participants will ingest 100grams of peanuts and a dual sugar solution during rest (day 1) or with following exercise (day 2, 75%Wmax cycling). A recently developed ELISA method will be used to assess the effect of exercise on circulating levels of Ara h6 after peanut intake and intestinal permeability will be determined.
Doel van het onderzoek
With a recently developed Ara h6 ELISA we would like to show the effect of exercise on circulating Ara h6 after peanut consumption
Onderzoeksopzet
Blood is sampled at baseline and at T=30, 60, 90, 120, and 240min after peanut consumption. Levels of Ara h6 will be analysed in serum samples collected at each of the time points with the sandwich ELISA method. Plasma levels of lactulose and rhamnose will be analysed in plasma samples collected at baseline and T=60min after peanut consumption. I-FABP will be measured in plasma samples collected at baseline and at T=30 and T=60 min after peanut consumption.
Onderzoeksproduct en/of interventie
Intake of 100grams of roasted peanuts during 2 test days
Intake of 5g Lactulose and 1gr L-rhamnose during two test days
Blood sampling via venflon cannula at baseline and 30, 60, 90, 120, and 240 min after peanut intake during two test days.
Cycling on ergometer 1hr 75% Wmax during 1 test day
Publiek
Lonneke Janssen Duijghuijsen
P.O. Box 17
Wageningen 6700 AA
The Netherlands
0317-482643
lonneke.janssen@wur.nl
Wetenschappelijk
Lonneke Janssen Duijghuijsen
P.O. Box 17
Wageningen 6700 AA
The Netherlands
0317-482643
lonneke.janssen@wur.nl
Belangrijkste voorwaarden om deel te mogen nemen (Inclusiecriteria)
- 20-35 year old males and females
- Body mass index (BMI) 23-27.5 kg/m2
- Suitable veins for blood sampling
- No inhibitory matrix effect in Ara h6 ELISA method
- Able to cycle 1h on ergometer
Belangrijkste redenen om niet deel te kunnen nemen (Exclusiecriteria)
- History of peanut allergy
- Known symptoms of immune disease, such as diabetes, gastritis, and coeliac disease.
- Pregnant or lactating
- Known symptoms of intestinal disease, such as Crohn’s Disease, ulcerative colitis, and irritable bowel syndrome.
- Smoking
- Use of hard drugs
- Use of specific medicines:
o Chronic use of NSAIDs: aspirins, ibuprofen, etc.
o Drugs having an effect on gastric and/or intestinal function and motility, including antidepressants.
- Participation in other scientific studies
- Blood donation during the last six weeks before the start and during the study
Opzet
Deelname
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In overige registers
| Register | ID |
|---|---|
| NTR-new | NL5701 |
| NTR-old | NTR5854 |
| Ander register | NL57339.081.16 : 16/11 METC Wageningen Universiteit |