Stromal Vascular Fraction injectable as a preventive treatment of scars.

In this prospective study we investigate the potential effect of Stromal Vascular Fraction (SVF)

injection as a preventive treatment of scar formation after mamma reduction. All patients will receive

SVF (0,1 ml per 0,5 cm2 scar surface) in 1 scar of 1 breast and 0,9% NaCL (0,1 ml per 0,5 cm2 scar

surface) in the other scar of the breast. Only the lateral side (till 5 cm from the edge) of the horizontal

scar will be treated and investigated in both breasts. SVF will be created by using a mechanical

dissociation procedure named: the FAT (Fractionation of Adipose Tissue) procedure. Postoperative

clinical improvement is measured with the POSAS questionnaire containing the observer scar

assessment and the patient scar assessment and will focus on: vascularization, pigmentation,

thickness, relief, pliability, pain, itchiness, stiffness and irregularity. 1 questionnaire (patient and

observer assessment) is completed for each breast. Postoperative scar improvement is also

measured by RNA isolation and histological observation of biopsies (into the dermal fat layer) on fixed

positions. Histological observation will focus on extracellular matrix remodeling, epidermis renewal,

cell infiltration and new microvasculature formation. RNA isolation will focus on pro- and anti-

inflammatory genes. 1 biopsy is taken of each breast every time. Postoperative improvement is

analyzed by a photographic panel assessment. Photographic evaluation will focus on scar size

reduction, change of color and relief using a Visual Analogue Scale (VAS).1 photograph is taken of

each breast every time. All measurements will be done on predetermined time-points: 6 months and

12 months postoperative, only the biopsies will be taken preoperative as well.

Stromal Vascular Fraction injection in the wound edges will prevent scar formation after a mammae reduction compared to a placebo injection.

Preoperative, 6 months and 12 months postoperative.

Stromal Vascular Fraction injection obtained by mechanical dissociation of adipose tissue. 1 ml of Stromal Vascular Fraction will be injected in the lateral 5 cm of the horizontal scar after a mamma reduction.