We hypothesize that cooling reduces endotoxemia induced inflammation and does not cause immunosuppression.
ID
Bron
Verkorte titel
Aandoening
Cooling, fever control, endotoxemia, inflammation, healthy volunteers
Ondersteuning
Onderzoeksproduct en/of interventie
Uitkomstmaten
Primaire uitkomstmaten
Host immune response: Il-6 (primary outcome), but also Full blood count, leukocyte differentiation, C-reactive protein (CRP)
Other pro- and anti-inflammatory cytokines by enzyme-linked immunosorbent assay (ELISA), markers of endothelial condition (von willebrand factor(vWF), syndecan-1, thrombomodulin by ELISA. Expression of granulocyte activation and adhesion molecules by flow cytometry. HLA-DR expression will aslo be measured with flow cytometry. Urine levels of catecholamines will be measured using ELISA. Whole blood ex-vivo stimulation with LPS and LTA
Doel van het onderzoek
We hypothesize that cooling reduces endotoxemia induced inflammation and does not cause immunosuppression.
Onderzoeksopzet
T=0, T=1, T=3, T=6, T=8
Onderzoeksproduct en/of interventie
Group 1: LPS + external cooling to 36¡ãC from T = 0 hours after LPS infusion
Group 2: LPS
Publiek
Matthew
Harmon
Meibergdreef 9,
1105AZ
+31205668222
Wetenschappelijk
Matthew
Harmon
Meibergdreef 9,
1105AZ
+31205668222
Belangrijkste voorwaarden om deel te mogen nemen (Inclusiecriteria)
1. Healthy male volunteer
2. Age ¡Ý 18 years <35 years
3. BMI between 20 and 25 kg/m2
Belangrijkste redenen om niet deel te kunnen nemen (Exclusiecriteria)
1. No informed consent
2. Any abnormal test result during the screening prior to inclusion of the study (medical history, physical examination, ECG, blood examination).
3. History of drug abuse
4. Any present medication use on prescription
5. Participation in any other medical drug study < 3 months
6. Participation in previous volunteer studies using LPS
7. History of an allergic reaction to opiates
Opzet
Deelname
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In overige registers
| Register | ID |
|---|---|
| NTR-new | NL6203 |
| NTR-old | NTR6367 |
| CCMO | NL53460.018.15 |
| OMON | NL-OMON45163 |