We hypothesize that inhalation of helium gas induces release of protective factors in the blood, which can be used to protect different cell types against ischemia/reperfusion injury.
ID
Bron
Verkorte titel
Aandoening
Ischemia/reperfusion injury
ischemie/reperfusie schade
Ondersteuning
Department of Anesthesiology, Academic Medical Center Amsterdam
Onderzoeksproduct en/of interventie
Uitkomstmaten
Primaire uitkomstmaten
- Cell damage such as LDH, apoptosis markers <br>
- Expression of Cav-1 and Cav-3 in blood<br>
Achtergrond van het onderzoek
Helium induces organ protection, but currently its mechanisms of action are unclear. This trial investigates the underlying mechanisms of helium-induced protection. We hypothesize that helium inhalation results in the release of "protective" mediators in the blood, which are transported to tissue at risk for ischemia/reperfusion damage. This is investigated in the current study in which healthy volunteers will breathe helium, after which blood will sampled. Different cells exposed to hypoxia/reoxygenation in vitro, will be incubated with this blood, and we will investigate whether a reduction in cell damage can be found.
Doel van het onderzoek
We hypothesize that inhalation of helium gas induces release of protective factors in the blood, which can be used to protect different cell types against ischemia/reperfusion injury.
Onderzoeksopzet
timepoints blood sampling: T0 (baseline), T1 (directly after inhalation), T2 (6 h after inhalation), T3 (24 h after inhalation)
Onderzoeksproduct en/of interventie
- Inhalation of helium or air
- Blood sampling by venous puncture
Publiek
Wetenschappelijk
Belangrijkste voorwaarden om deel te mogen nemen (Inclusiecriteria)
non-smoking, healthy male volunteers, aged 20-55 years
Belangrijkste redenen om niet deel te kunnen nemen (Exclusiecriteria)
-Active smoking or smoking in the previous six months
-Alcohol abuse or use of recreational drugs
-Any allergic reaction on medication in the past
-Presence of a chronic disease that is under current medical observation and needs pharmacological treatment, e.g. asthma, high blood pressure or diabetes
Opzet
Deelname
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Andere (mogelijk minder actuele) registraties in dit register
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In overige registers
| Register | ID |
|---|---|
| NTR-new | NL4367 |
| NTR-old | NTR4507 |
| Ander register | METC nummer : 2014_045 |
Samenvatting resultaten
preconditioning in the human endothelium in vivo. Anesthesiology 2013 Jan;118(1):95-104
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Oei GTML, Smit KF, van de Vondenvoort D, Wieland CW, Hollmann MW, Preckel B, Weber NC; The effect of helium inhalation on the innate and early adaptive immune system ex vivo. J Transl Med 2012;10:201
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Oei GTML, Huhn R, Heinen A, Hollmann MW, Schlack WS, Preckel B, Weber NC: Helium-induced cardioprotection of healthy and hypertensive rat myocardium in vivo. Eur J Pharmacol 2012; 684:125-131
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Berger MM, Huhn R, Oei GTML, Heinen A, Winzer A, Preckel B, Weber NC, Schlack W, Hollmann MW: Hypoxia induces late preconditioning in the rat heart in vivo. Anesthesiology 2010; 113:1351-60
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Oei GTML, Weber NC, Hollmann MW, Preckel B: Cellular effects of helium in different organs. Anesthesiology 2010; 112:1503-10.
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Huhn R HA, Weber NC, Kerindongo R, Oei GTML, Hollmann MW, Schlack W, Preckel B: Helium-induced early preconditioning and postconditioning is abolished in obese Zucker rats in vivo. J Pharmacol Exp Ther 2009; 329:600-7