MSOT beeldvorming in de onderbenen van gezonde vrijwilligers.

The current project comprises a proof of principal study to test the technical feasability of the use of Multispectral Optoacoustic Tomography (MSOT) in healthy volunteers for non-invasive measurements and imaging of perfusion and oxygenation in the lower legs. MSOT is a non-invasive imaging modality that provides real-time quantifiable in vivo visualizations dependent on the intrinsic difference in optical absorption properties of tissues. MSOT uses pulsed laser light of various wavelengths to excitate the molecules in tissue. The excitated molecules generate a ultrasonic wave, which is detected by an ultrasonic detector. Unlike optical microscopy, optoacoustic imaging is not influenced significantly by light scattering in tissue, because it detects ultrasonic waves. This results in the ability to maintain a high resolution and quantifiability even at depths of 1.5-2 cm. The imaging system is essentially a laser and an ultrasound detector combined in a handheld probe which is connected to a computer for processing, and a display for real-time video-rate visualisation. By measuring peripheral blood oxygenation and perfusion with a prototype handheld MSOT probe and comparing that to standard ultrasound imaging, pulse-oximetry and standard ankle-brachial index as a gold standard, the diagnostic accuracy and measurement ranges in physiologic conditions as determined by MSOT will be tested.

Evalueren wat de gevoeligheid van de MSOT techniek is voor het meten van de doorbloeding en de hoeveelheid zuurstof in de onderbenen van gezonde vrijwilligers

One time-point measurement after obtaining informed consent

Measurement variation (mean and SD) of MSOT in the lower leg.
Correlation of MSOT oxygenation and perfusion measurements with standard ultrasound imaging of the fixed anatomical landmarks, pulse-oximetry data for oxygenation status at the hallux and the ankle-brachial index of the right lower leg.