In this study we aim to investigate whether intestinal acetate production by gutmicrobiota is related to (postprandial) plasma acetate levels, insulin secretion/sensitivity and the CNS regulation of food intake (fMRI).
ID
Bron
Verkorte titel
Aandoening
We will include subjects from the Amsterdam HELIUS study (n=6000) and include only those with 10% lowest and 10% highest fecal butyryl-coenzyme A transferase enzyme (based on previously obtained results of 16S analysis and cross-validated by qPCR butyryl CoA transferase) will be approached for this study.
Ondersteuning
Onderzoeksproduct en/of interventie
Uitkomstmaten
Primaire uitkomstmaten
- differences in Postprandial plasma acetate levels after a SMMT<br>
- differences in Baseline Intestinal microbiota composition (morning stool samples)<br>
- differences in baseline Faecal acetate levels
Achtergrond van het onderzoek
We designed a comparative study to investigate whether a high intestinal acetate production is associated with altered acetate production and whether this affects insulin sensitivity and secretion as well as with neural responses to virtual and actual food stimuli (fMRI).
Doel van het onderzoek
In this study we aim to investigate whether intestinal acetate production by gutmicrobiota is related to (postprandial) plasma acetate levels, insulin secretion/sensitivity and the CNS regulation of food intake (fMRI).
Onderzoeksopzet
1 day
Onderzoeksproduct en/of interventie
(high fiber) containing standardized mixed meal test
Publiek
MEIBERGDREEF 9, KAMER F4.159.2
M. Nieuwdorp
Amsterdam 1105 AZ
The Netherlands
+31 (0)20 5666612
m.nieuwdorp@amc.uva.nl
Wetenschappelijk
MEIBERGDREEF 9, KAMER F4.159.2
M. Nieuwdorp
Amsterdam 1105 AZ
The Netherlands
+31 (0)20 5666612
m.nieuwdorp@amc.uva.nl
Belangrijkste voorwaarden om deel te mogen nemen (Inclusiecriteria)
- Male or female
- Stable bodyweight (<5% reported weight change during the previous 3 months)
- Age 18-65 years
- Caucasian (i.e.: Dutch or Turkish nationality)
- Able and willing to give informed consent
Belangrijkste redenen om niet deel te kunnen nemen (Exclusiecriteria)
- Use of corticosteroids, antibiotics and other immunomodulating medication in last 3 months
- XTC, amphetamine, nicotine or cocaine abuse, based on the 20 item drug abuse screening test (DAST-20)
- Alcohol abuse (> 3 units per day)
- Contraindication for MRI (neurological illness , malignancy, history of major heart
disease, history of major renal disease, pregnancy or breast feeding, pacemaker and
metals contraindicated for MRI present in the body)
- Expected prolonged compromised immunity (due to recent cytotoxic chemotherapy or
- HIV infection with a CD4 count <240)
- Presence of inflammatory bowel disease (IBD)2
- Inability to understand the study protocol, give informed consent or participate
adequately in study protocol
Opzet
Deelname
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Andere (mogelijk minder actuele) registraties in dit register
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In overige registers
| Register | ID |
|---|---|
| NTR-new | NL7131 |
| NTR-old | NTR7328 |
| Ander register | : METC 2018_053 |