The study investigates if how the skin recovers after barrier disruption by tape-stripping and what the effects the application of a formulation is on this process. Lipophilic formulations are commonly used in the treatment of atopic eczema, yet,…
ID
Bron
Verkorte titel
Aandoening
Atopic Eczema, skin barrier, stratum corneum
Onderzoeksproduct en/of interventie
Uitkomstmaten
Primaire uitkomstmaten
The lipid compostion of the stratum corneum. The lameller and lateral lipid organization.
Achtergrond van het onderzoek
Information not provided by researcher.
Doel van het onderzoek
The study investigates if how the skin recovers after barrier disruption by tape-stripping and what the effects the application of a formulation is on this process. Lipophilic formulations are commonly used in the treatment of atopic eczema, yet, how these formulations exert there effect is unknown. In this study the effects of the formulation on the barrier function measured as trans epidermal water loss and the lipid composition and organization of the other most layer of the skin: the stratum corneum, are investigated. It is hypothesized that the formulation will affect the lipid composition of the stratum corneum and this changes towards a composition with better barrier properties thereby improve the skin barrier.
Onderzoeksopzet
The trans epidermal water loss will be monitored at day 0, 1, 2, 3, 7, and 16 after barrier disruption. The lipid composition is measured by acquiring stratum corneum material at day 16 by tape stripping. Tape strips are extracted and analyzed using liquid chromatography and mass spectrometry. The lateral organization is studied at day 16 using attenuated total reflectance Fourier-transform infrared spectroscopy. The lamellar organization is studied using small angle x-ray diffraction of biopsy obtained at day 16.
Onderzoeksproduct en/of interventie
Treatment with a venix caseosa based formulation
Publiek
[default]
The Netherlands
Wetenschappelijk
[default]
The Netherlands
Belangrijkste voorwaarden om deel te mogen nemen (Inclusiecriteria)
- Age between 18-40
- Caucasian
Belangrijkste redenen om niet deel te kunnen nemen (Exclusiecriteria)
- Abundant hair presence on the ventral forearms;
- Unnatural abnormalities on one of their ventral forearms (e.g. skin lesions, tattoos);
- Subjects using any systemic drug therapy (e.g. cholesterol-lowering drugs, insulin
related drugs, steroids and immunosuppressants);
- Chronically inflammatory disease;
- Dermatological disorders or a history of dermatological disorders;
- History of drug abuse;
- Pregnancy;
Opzet
Deelname
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In overige registers
| Register | ID |
|---|---|
| NTR-new | NL7003 |
| NTR-old | NTR7193 |
| CCMO | NL51870.058.14 |
| OMON | NL-OMON43983 |