The main aim of the current study will be to assess the effect of acute blood flow restriction and blood flow restriction combined with resistance exercise on the muscle protein synthetic rate in healthy young male subjects
ID
Source
Brief title
Condition
- Other condition
Synonym
Health condition
Effect van beperking van de bloedtoevoer naar spieren in rust en tijdens inspanning op de spiereiwit opbouw
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
The main study endpoint will be the 5 h muscle protein synthesis (MPS) rate
Secondary outcome
The early (0-2h) and late (2-5h) muscle protein synthesis
Background summary
The combined application of blood flow restriction and low load resistance
exercise has been found to stimulate muscle protein synthetic rate to a similar
extent as the traditional high load resistance exercise without blood flow
restriction. However, it is still unclear whether blood flow restriction alone
(without exercise) is a strong enough stimulus to significantly increase muscle
protein synthesis.
Study objective
The main aim of the current study will be to assess the effect of acute blood
flow restriction and blood flow restriction combined with resistance exercise
on the muscle protein synthetic rate in healthy young male subjects
Study design
The present study will use a randomized, open labelled parallel study design.
Intervention
Two acute cycles of blood flow restriction of a lower extremity with or without
low load resistance exercise
Study burden and risks
The subjects will participate in 1 screening and 1 test day. They will be
required to fill out a medical questionnaire during the screening session. A
part of the subjects will also be asked to perform a 1-repetition maximum test
during the screening session. They will receive a venous catheter that will be
used for repeated blood draws during the test day and an amino-acid infusion
that will stay in place until the test day has been completed. The subjects
will be subjected to blood flow restriction in rest, or while performing
resistance exercise, After this intervention, subjects will be monitored during
a period of 5 hours in rest in which 3 biopsies from each leg will be taken.
Subjects will be instructed to refrain from intense physical activity in the 48
h prior to the test day, and to avoid consumption of alcohol and caffeine in
drinks in the 24 and 12 hours prior to the test day, respectively. Subjects
will also be asked to avoid eating and drinking anything (except for water)
from 22:00h the evening before a test day. There are no major risks of short
term restriction of blood flow to a muscle except for the fact that the
pressure of the cuff when inflated can cause some discomfort. Furthermore the
risks of performing restriction exercise are also minimal and and comparably to
the expected risks associated with exercise performance (such as fatigue,
fainting and an abnormal blood pressure)
Universiteitssingel 50
Maastricht 6229 ER
NL
Universiteitssingel 50
Maastricht 6229 ER
NL
Listed location countries
Age
Inclusion criteria
- Males
- Aged between 18-35 years
- Healthy
- 18.5<=BMI <=30 kg/m2
Exclusion criteria
- Smoking
- Resistance exercise >1 session/week
- Sports/exercise >3 session/week
- Lactose intolerant
- A history of neuromuscular problems
- Recent (<1 y) participation in amino acid tracer studies
- Individuals on any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription acne medications).
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
In other registers
| Register | ID |
|---|---|
| CCMO | NL56003.068.15 |
| OMON | NL-OMON24024 |